The Ladykillers (2004)

Directed by: Ethan Coen, Joel Coen

Cast: Tom Hanks, Marlon Wayans and Irma P. Hall

 IMDb page about The Ladykillers

Studio(s): Universal Studios Hollywood

The house featured throughout is the Harvey house, on Colonial Street on the Universal Studios backlot.
More about the Harvey house.

Trailer: